15-min Cities: the Potential of a Medium-Sized Polycentric Latin American City.

Urban sustainability and sustainable mobility have become the central focus of sustainable development initiatives. The city of 15 models seeks to ensure that urban development is sustainable. This paper evaluates the current state of mobility and the use of sustainable transport in the specific context of the city of Antofagasta, which, due to its characteristics of urban expansion and its mining activity, has been considered an international reference case. In particular, we study how the current urban structure shapes the city's travel patterns and identify opportunities for public policy actions to focus their investment and urban intervention efforts on the essential aspects that make it possible to achieve 15- or 20-min territories. The results reveal the need for urban development initiatives to focus on improving the supply of goods and services in areas with low installed capacity, promoting the culture of micro-neighbourhoods, and encouraging cycling. In particular, only 10% of trips, for all purposes and all modes of transport, are completed in less than 15 min. And about 58% of trips, for all purposes and all modes, are completed in less than 30 min. In the marginal effects of the multinomial logit model, a one-unit increase in travel time increases the probability of choosing to travel by public transport by 63% and decreases the probability of walking by 41%.

Substitutions in Spike and Nucleocapsid proteins of SARS-CoV-2 circulating in South America.

SARS-CoV-2 is a new member of the genus Betacoronavirus, responsible for the COVID-19 pandemic. The virus crossed the species barrier and established in the human population taking advantage of the spike protein high affinity for the ACE receptor to infect the lower respiratory tract. The Nucleocapsid (N) and Spike (S) are highly immunogenic structural proteins and most commercial COVID-19 diagnostic assays target these proteins. In an unpredictable epidemic, it is essential to know about their genetic variability. The objective of this study was to describe the substitution frequency of the S and N proteins of SARS-CoV-2 in South America. A total of 504 amino acid and nucleotide sequences of the S and N proteins of SARS-CoV-2 from seven South American countries (Argentina, Brazil, Chile, Ecuador, Peru, Uruguay, and Colombia), reported as of June 3, and corresponding to samples collected between March and April 2020, were compared through substitution matrices using the Muscle algorithm. Forty-three sequences from 13 Colombian departments were obtained in this study using the Oxford Nanopore and Illumina MiSeq technologies, following the amplicon-based ARTIC network protocol. The substitutions D614G in S and R203K/G204R in N were the most frequent in South America, observed in 83% and 34% of the sequences respectively. Strikingly, genomes with the conserved position D614 were almost completely replaced by genomes with the G614 substitution between March to April 2020. A similar replacement pattern was observed with R203K/G204R although more marked in Chile, Argentina and Brazil, suggesting similar introduction history and/or control strategies of SARS-CoV-2 in these countries. It is necessary to continue with the genomic surveillance of S and N proteins during the SARS-CoV-2 pandemic as this information can be useful for developing vaccines, therapeutics and diagnostic tests.

Molecular analysis of several in-house rRT-PCR protocols for SARS-CoV-2 detection in the context of genetic variability of the virus in Colombia.

The COVID-19 pandemic caused by SARS-CoV-2 is a public health problem unprecedented in the recent history of humanity. Different in-house real-time RT-PCR (rRT-PCR) methods for SARS-CoV-2 diagnosis and the appearance of genomes with mutations in primer regions have been reported. Hence, whole-genome data from locally-circulating SARS-CoV-2 strains contribute to the knowledge of its global variability and the development and fine tuning of diagnostic protocols. To describe the genetic variability of Colombian SARS-CoV-2 genomes in hybridization regions of oligonucleotides of the main in-house methods for SARS-CoV-2 detection, RNA samples with confirmed SARS-CoV-2 molecular diagnosis were processed through next-generation sequencing. Primers/probes sequences from 13 target regions for SARS-CoV-2 detection suggested by 7 institutions and consolidated by WHO during the early stage of the pandemic were aligned with Muscle tool to assess the genetic variability potentially affecting their performance. Finally, the corresponding codon positions at the 3' end of each primer, the open reading frame inspection was identified for each gene/protein product. Complete SARS-CoV-2 genomes were obtained from 30 COVID-19 cases, representative of the current epidemiology in the country. Mismatches between at least one Colombian sequence and five oligonucleotides targeting the RdRP and N genes were observed. The 3' end of 4 primers aligned to the third codon position, showed high risk of nucleotide substitution and potential mismatches at this critical position. Genetic variability was detected in Colombian SARS-CoV-2 sequences in some of the primer/probe regions for in-house rRT-PCR diagnostic tests available at WHO COVID-19 technical guidelines; its impact on the performance and rates of false-negative results should be experimentally evaluated. The genomic surveillance of SARS-CoV-2 is highly recommended for the early identification of mutations in critical regions and to issue recommendations on specific diagnostic tests to ensure the coverage of locally-circulating genetic variants.

Efecto de la empatía afectiva sobre el procesamiento cortical de emojis / Effect of Affective Empathy on the Cortical Processing of Emojis / Effect of Affective Empathy on the Cortical Processing of Emojis

Resumen Objetivo. Identificar las diferencias en el procesamiento cortical de emojis en personas con alta y baja empatía afectiva. Método. El estudio se llevó a cabo con 69 participantes, distribuidos en dos grupos -baja y alta empatía afectiva-, según su puntaje en el Índice de Reactividad Interpersonal. Cada participante observó emojis con expresiones de alegría, ira y neutros. Se registraron los potenciales relacionados a eventos (PRE) P100, N170 y LPP. Resultados. Se encontró una mayor amplitud del N170 en el grupo de personas con alta empatía afectiva y, en general, mayor magnitud del LPP ante emojis de ira. Conclusión. Los resultados sugieren que los emojis son procesados corticalmente de forma similar al rostro humano y que la empatía afectiva modula la codificación del emoji, pero no la atención temprana y el enganche atencional hacia estos estímulos.

Abstract Objective. The objective of this research was to identify the differences in the cortical processing of emojis in people with high and low affective empathy. Method. The study was carried out with 69 participants divided into two groups, low and high affective empathy, according to their score in the Interpersonal Reactivity Index. Each participant observed emojis with happy, neutral and angry expressions. Events related potential (ERP) P100, N170 and LPP were recorded. Results. An amplitude greater than N170 in the high affective empathy group, and a generally greater magnitude of the LPP in response to angry emoji were found. Conclusion. The results suggest that the emojis are processed cortically in a similar way to the human face and that affective empathy modulates the coding of the emoji but not the early attention and the attentional attachment to these stimuli.

Resumo Escopo. Identificar as diferencias no processamento cortical de emojis em pessoas com alta e baixa empatia afetiva. Metodologia. O estudo foi feito com 69 participantes, distribuídos em dois grupos, baixa e alta empatia afetiva, segundo sua pontuação no Índice de Reatividade Interpessoal. Cada participante observou emojis com expressões de alegria, neutrais e ira. Foram registrados os potenciais relacionados a eventos (PREs) P100, N170 e LPP. Resultados. Foi achada uma maior amplitude do N170 em um grupo de pessoas com alta empatia afetiva e, em geral, maior magnitude do LPP frente a de emoji com ira. Conclusão. Os resultados sugerem que os emojis são processados corticalmente de forma similar ao rosto humano e que a empatia afetiva modula a codificação do emoji mas não a atenção precoce e o engajamento atencional para estes estímulos.

Treatment of cutaneous actinomycosis with amoxicillin/clavulanic acid.

OBJECTIVE: To evaluate the efficacy and tolerability of amoxicillin/clavulanic (AMX/CLV) acid as treatment for cutaneous actinomycosis. METHODS: We present a long-term follow-up study of cutaneous actinomycosis patients. Cervicofacial (CFA) and abdominal (AA) were recruited during 6 years. Diagnoses were based on clinical and microbiological characteristics; presence of granules, isolation and identification of etiological agents were carried out in each case. Patients received AMX-CLV 875/125 mg BID PO at a maximum period of 12 weeks. RESULTS: Twenty-two cases were enrolled; the mean age was 45.2 years old. Twenty patients (91%) presented CFA and two AA (9%). All patients with CFA had dental caries, seven (35%) with periodontal disease and 10 (50%) had type-2 diabetes mellitus (T2DM). One case of AA had history of intrauterine device and other appendicitis. Granules were observed in all the cases, the main etiological agent was Actinomyces israelii 16/22 (72.7%). Clinical and microbiological cure was achieved in 19/22 cases (86.4%), the remaining patients presented clinical improvement. The average duration of the treatment was 6.6 weeks. Side effects were recorded in 4/19 cases (18.2%), three of them presented nausea and one diarrhea. CONCLUSION: Treatment with AMX/CLV acid showed efficacy in the management of actinomycosis with cutaneous involvement.

Superficial Mycoses Associated with Diaper Dermatitis.

Diapers create particular conditions of moisture and friction, and with urine and feces come increased pH and irritating enzymes (lipases and proteases). Fungi can take advantage of all these factors. Candida yeasts, especially C. albicans, are responsible for the most frequent secondary infections and are isolated in more than 80 % of cases. Correct diagnosis is important for ensuring the correct prescription of topical antimycotics. Nystatin, imidazoles and ciclopirox are effective. It is important to realize there are resistant strains. Dermatophytes can infect the diaper area, with the most common agent being Epidermophyton floccosum. The clinical characteristics of dermatophytosis are different from those of candidiasis, and it can be diagnosed and treated simply. Malassezia yeasts can aggravate conditions affecting the diaper area, such as seborrheic dermatitis, atopic dermatitis, and inverse psoriasis. Additional treatment is recommended in this case, because they usually involve complement activation and increased specific IgE levels. Erythrasma is a pseudomycosis that is indistinguishable from candidiasis and may also occur in large skin folds. It is treated with topical antibacterial products and some antimycotics.

Periungual eccrine poroma.

BACKGROUND: Eccrine poroma is a rare, benign skin appendage tumor originating from the intraepidermal portion of the eccrine sweat duct, which typically occurs on the sides and soles of the feet. Nonetheless, eccrine poroma may be found in any skin area bearing sweat glands. OBJECTIVE: Herein we report a case of an eccrine poroma in an unusual location, the surgical management of the condition, and follow-up processes.

Periungual eccrine poroma.

BACKGROUND: Eccrine poroma is a rare, benign skin appendage tumor originating from the intraepidermal portion of the eccrine sweat duct, which typically occurs on the sides and soles of the feet. Nonetheless, eccrine poroma may be found in any skin area bearing sweat glands. OBJECTIVE: Herein we report a case of an eccrine poroma in an unusual location, the surgical management of the condition, and follow-up processes.

Mucormycosis in children: a study of 22 cases in a Mexican hospital.

We present a single-centre, retrospective study (1985-2012) of 22 cases of mucormycosis in children. A total of 158 mucormycosis cases were identified, of which 22 (13.96%) were children. The mean age of the children was 10.3 years (range: 6 months-18 years), and 59% of the infections occurred in males. The rhinocerebral form was the main clinical presentation (77.27%), followed by the primary cutaneous and pulmonary patterns. The major underlying predisposing factors were diabetes mellitus in 68.18% of the patients and haematologic diseases in 27.7% of the patients. The cases were diagnosed by mycological tests, with positive cultures in 95.4% of the patients. Rhizopus arrhizus was the foremost aetiologic agent in 13/22 cases (59.1%). In 21 cultures, the aetiologic agents were identified morphologically and by molecular identification. In 10 cultures, the internal transcribed spacer region of the ribosomal DNA was sequenced. Clinical cure and mycological cure were achieved in 27.3% cases, which were managed with amphotericin B deoxycholate and by treatment of the underlying conditions.

Mycetoma: experience of 482 cases in a single center in Mexico.

Mycetoma is a chronic granulomatous disease. It is classified into eumycetoma caused by fungi and actinomycetoma due to filamentous actinomycetes. Mycetoma can be found in geographic areas in close proximity to the Tropic of Cancer. Mexico is one of the countries in which this disease is highly endemic. In this retrospective study we report epidemiologic, clinical and microbiologic data of mycetoma observed in the General Hospital of Mexico in a 33 year-period (1980 to 2013). A total of 482 cases were included which were clinical and microbiology confirmed. Four hundred and forty four cases (92.11%) were actinomycetomas and 38 cases (7.88%) were eumycetomas. Most patients were agricultural workers; there was a male predominance with a sex ratio of 3:1. The mean age was 34.5 years old (most ranged from 21 to 40 years). The main affected localization was lower and upper limbs (70.74% and 14.52% respectively). Most of the patients came from humid tropical areas (Morelos, Guerrero and Hidalgo were the regions commonly reported). The main clinical presentation was as tumor-like soft tissue swelling with draining sinuses (97.1%). Grains were observed in all the cases. The principal causative agents for actinomycetoma were: Nocardia brasiliensis (78.21%) and Actinomadura madurae (8.7%); meanwhile, for eumycetomas: Madurella mycetomatis and Scedosporium boydii (synonym: Pseudallescheria boydii) were identified. This is a single-center, with long-follow up, cross-sectional study that allows determining the prevalence and characteristics of mycetoma in different regions of Mexico.

La enseñanza virtual en el contexto de la educación actual / Virtual education in current education

La enseñanza virtual ha venido incursionando en forma progresiva en la educación actual, principalmente como método de formación y actualización en la población adulta después de graduada. Sus características exigen un replanteamiento de las funciones tanto de los alumnos como de los docentes. Este artículo pretende, por un lado, evaluar las similitudes y diferencias entre la enseñanza presencial y la virtual, y, por otro, mostrar algunas de las habilidades requeridas por los docentes para realizarla.

Factores de riesgo de infeccion por Pseudomona Aeruginosa en el servicio de quemados del hospital Simon Bolivar de Bogota 1986 / Risk factors for infection due to Pseudomona Aeruginosa in Hospital Simon Bolivar burn's ward. Bogota 1986

La presente investigacion se planeo y se ejecuto, partiendo de la base estadistica del problema; se ubico por experiencia personal, por observacion directa y por criterios existentes en el servicio de quemados, los diez factores comunes de riesgo, para poder hacer un analisis comparativo en el manejo del paciente. Una vez descritos estos factores comunes de riesgo , se planeo la toma, recoleccion y tabulacion de la informacion necesaria para el logro de nuestros objetivos. Luego de recolectadas y procesadas las muestras, se tabulo por medios manuales y mecanicos los resultados, para hacer los analisis y las conclusiones descritas anteriormente. Concluida la investigacion, se comenzo a buscar las estrategias adecuadas para encontrar los mecanismos de control de esta cadena epidemiologica demostrada para su aplicabilidad..